The 2-Minute Rule for megatomi.com
The 2-Minute Rule for megatomi.com
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Our new Apparent+ tissue clearing method is the sole approach that delipidates samples without having alter in morphology and with nominal effect on structural integrity.
Megatome is usually a vibrating microtome designed to section a broad choice of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With higher blade vibrating frequency and minimized blade deflection, Megatome allows large-throughput tissue sectioning with uniform floor profile, together with negligible tissue harm and data reduction.
Antibodies could get months to diffuse by only some millimeters of tissue, that has a steep labeling gradient from area to Main.
Working the CLI directly from a Gradle job isn't presently supported. A distribution should be created via gradlew :j2d-cli:distZip to produce a zip file made up of every thing needed to run.
SE utilizes a rotational electrical discipline to disperse very electromobile molecules (like antibodies or surfactant micelles) all over a porous sample with no detrimental electrically charged structures within the tissue. This allows two-four day clearing of intact organs,
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eFLASH is really a speedy tissue labeling method that allows for uniform full-organ staining in twenty rounds of labeling.
The SmartSPIM light-weight sheet microscope offers unparalleled resolution and acquisition pace during intact tissue volumes, while its modular structure helps you to upgrade and customise unique factors.
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SHIELD avoids the variability of hydrogel embedding and the data reduction from PFA preservation, safeguarding specimens for several rounds of processing.
Leverage the Very clear+ tissue clearing strategy, coupled with eFLASH and patented stochastic electrotransport technologies, to swiftly obvious and label complete organs. Important highlights and options include:
Our novel SHIELD tissue preservation approach varieties intramolecular bonds applying polyfunctional, adaptable epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.